Beta Catenin Antibody (R32807)

Western blot testing of 1) human placenta, 2) human A431, 3) human SK-OV-3, 4) rat heart and 5) mouse testis lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

IF/ICC staining of FFPE human A431 cells with Beta Catenin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry testing of human A549 cells with Beta Catenin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Catenin antibody.

IHC testing of FFPE human breast cancer tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human prostate cancer tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human liver cancer tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE mouse liver tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE mouse small intestine tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat liver tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat small intestine tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat spleen tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat heart tissue with Beta Catenin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Western blot testing of human 1) placenta, 2) PC-3, 3) U-87 MG, 4) Caco-2, 5) HeLa, 6) A549 and 7) K562 lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Western blot testing of 1) rat heart, 2) mouse heart, 3) mouse lung and 4) mouse liver lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Immunofluorescent staining of FFPE human intestine cancer tissue with Beta Catenin antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.