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Home >> Antibodies >> Beta Actin Antibody Loading Control

Beta Actin Antibody Loading Control (N1037)

  Catalog No Formulation Size Price (USD)  
N1037-100UG 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 275

N1037-25UG 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 25 ug 129
Western blot testing of human samples with beta Actin antibody at 1 ug/ml.
Western blot testing of mouse samples with beta Actin antibody at 1 ug/ml.
Species Reactivity Human, Mouse, Rat, Monkey, Rabbit, Chicken, Dog
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A Affinity Chromatography
Buffer 1X PBS, pH 7.4
Gene ID 60
Applications Western blot : 1-3ug/ml
Limitations This beta Actin antibody loading control is available for research use only.

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Description

Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Application Notes

Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.

Immunogen

A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.

Storage

Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

Alternate Names

Actin, WB loading control

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