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Home >> Antibodies >> Bcl6 Antibody

Bcl6 Antibody [clone BCL6/1527] (V3496)

  Catalog No Formulation Size Price (USD)  
V3496-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429

V3496-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V3496SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459

V3496IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
Protein array validation of the Bcl6 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Bcl6 antibody (clone BCL6/1527).

Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human tonsil tissue with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
IHC testing of FFPE human lymphoma with Bcl6 antibody (clone BCL6/1527). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name BCL6/1527
Purity Protein G affinity chromatography
UniProt P41182
Localization Nuclear
Applications Flow Cytometry : 0.5-1ug/million cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.125-0.25ug/ml
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Bcl6 antibody is available for research use only.

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Description

Antibody to Bcl-6 is helpful in a number of diagnostic settings: First, in the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show Bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. Second, Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, Bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Third, Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Application Notes

Optimal dilution of the Bcl6 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 256-389 were used as the immunogen for the Bcl6 antibody.

Storage

Store the Bcl6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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