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Home >> Antibodies >> Bcl-X Antibody

Bcl-X Antibody [clone BX006] (V2011)

  Catalog No Formulation Size Price (USD)  
V2011-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2011-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2011SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2011IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of Hodgkin's lymphoma stained with Bcl-X antibody (BX006). Note cytoplasmic and membrane staining.
Western blot testing of human samples.
SDS-PAGE Analysis of Purified, BSA-Free Bcl-X Antibody (clone BX006). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human, Mouse, Rat, and Pig. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name BX006
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 598
Localization Cytoplasmic and cell/nuclear membrane
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (3)
Limitations This Bcl-X antibody is available for research use only.

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Description

This antibody recognizes a protein of 27kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

Storage

Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

B cell lymphoma 2 like, BclXL, BclXS

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