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Home >> Antibodies >> Bcl-2 Antibody

Bcl-2 Antibody [clone HF-2] (RQ5323)

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Image RQ5323 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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IHC staining of FFPE human spleen tissue with Bcl-2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human spleen tissue with Bcl-2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat and 2) MCF-7 cell lysate with Bcl-2 antibody. Predicted molecular weight ~26 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name HF-2
Purity Affinity purified
UniProt P10415
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This Bcl-2 antibody is available for research use only.
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Description

The BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. [RefSeq]

Application Notes

Optimal dilution of the Bcl-2 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Bcl-2 was used as the immunogen for the Bcl-2 antibody.

Storage

Store the Bcl-2 antibody at -20oC.

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