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  • Email: info@nsjbio.com
Home >> Antibodies >> Bcl-2 Antibody

Bcl-2 Antibody [clone 8C8] (V2008)

  Catalog No Formulation Size Price (USD)  
V2008-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2008-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2008SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2008IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human tonsil stained with Bcl-2 antibody (8C8).Note cell membrane and cytoplasmic staining.
Western blot testing of human samples with Bcl-2 antibody (8C8).
Species Reactivity Human, Monkey and Pig. Does not react with Mouse and Rat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 8C8
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 596
Localization Cytoplasmic, membrane
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Bcl-2 antibody is available for research use only.

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Description

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Storage

Store the Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Apoptosis regulator Bcl-2, B-cell CLL / lymphoma-2, Bcl-2 antibody

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