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Home >> Antibodies >> Bcl-2 Antibody

Bcl-2 Antibody [clone 100/D5] (V2007)

  Catalog No Formulation Size Price (USD)  
V2007-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2007-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2007SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2007IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5). Note nuclear membrane & cytoplasmic staining.
Western blot testing of human samples using Bcl-2 antibody (100/D5).
FACS staining (intracellular) of Jurkat cells using Bcl-2 antibody (red) and isotype control (green).
Species Reactivity Human. Does not react with Mouse and Rat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 100/D5
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 596
Localization Cytoplasmic, membrane
Applications ELISA : order BSA-free format for coating
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunoprecipitation : 1-2ug/0.5mg lysate
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Bcl-2 antibody is available for research use only.

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Description

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.

Storage

Store the Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

B-cell CLL / lymphoma-2

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