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Home >> Antibodies >> b-Actin Antibody [Discontinued, view replacements]

b-Actin Antibody [Discontinued, view replacements] (R30971)

  Catalog No Formulation Size Price (USD)  
R30971 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 285
Western blot testing of b-Actin antibody and Lane 1: rat testis; 2: rat ovary; 3: rat heart; 4: rat brain; 5: A453; 6: HeLa; 7: MCF-7; 8: SMMC-7721; 9: SW620
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P60709
Applications Western blot : 0.5-1ug/ml
Limitations This b-Actin antibody is available for research use only.

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Description

b-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.

Application Notes

The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the b-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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