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Home >> Antibodies >> ASIC2 Antibody / ACCN1

ASIC2 Antibody / ACCN1 (RQ6038)

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  Catalog No Formulation Size Price (USD)  
Image RQ6038 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with ASIC2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with ASIC2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with ASIC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ASIC2 antibody.
Flow cytometry testing of human PC-3 cells with ASIC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ASIC2 antibody.
Western blot testing of human 1) placenta, 2) PC-3, 3) A549, 4) U-2 OS and rat 5) brain, 6) liver, 7) ovary and mouse 8) brain, 9) liver and 10) ovary lysate with ASIC2 antibody. Predicted molecular weight ~58 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q16515
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ASIC2 antibody is available for research use only.
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Description

Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2,is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.

Application Notes

Optimal dilution of the ASIC2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids H27-C512) was used as the immunogen for the ASIC2 antibody.

Storage

After reconstitution, the ASIC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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