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Home >> Antibodies >> Anti-EpCAM Antibody / Extracellular domain

Anti-EpCAM Antibody / Extracellular domain [clone EGP40/1372] (V3525)

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  Catalog No Formulation Size Price (USD)  
Image V3525-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3525-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3525SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3525IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated
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Immunofluorescent staining of FFPE human colorectal carcinoma with anti-EpCAM antibody (green) and Reddot nuclear stain (red).
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Immunofluorescent staining of FFPE human colorectal carcinoma with anti-EpCAM antibody (green) and Reddot nuclear stain (red).
Immunofluorescent staining of human MCF-7 cells with anti-EpCAM antibody (green, clone EGP40/1372) and Reddot nuclear stain (red).
Western blot testing of human 1) HEK293, 2) A431 and 3) HCT116 cell lysate with anti-EpCAM antibody (clone EGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human colorectal carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Western blot testing of human lung lysate with anti-EpCAM antibody (clone EGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Flow cytometry testing of PFA-fixed human MCF7 cells with anti-EpCAM antibody (clone EGP40/1372); Red=isotype control, Blue= anti-EpCAM antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using the anti-EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb.

Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free anti-EpCAM antibody (clone EGP40/1372) as confirmation of integrity and purity.
IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.
Availability 1-3 business days
Species Reactivity Human, Dog, Cat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name EGP40/1372
Purity Protein G affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Titering of the anti-EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.

Storage

Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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