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Home >> Antibodies >> Anti-ACTH Antibody

Anti-ACTH Antibody [clone SPM333] (V9067)

  Catalog No Formulation Size Price (USD)  
V9067-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V9067-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V9067SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
IHC testing of FFPE human pituitary gland with anti-ACTH antibody (clone SPM333).
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat. Expected to show a broad species reactivity.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM333
Purity Protein G affinity chromatography
UniProt P01189
Localization Cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Limitations This anti-ACTH antibody is available for research use only.

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Description

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Application Notes

The optimal dilution of the anti-ACTH antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Amino acids 1-24 of the human protein were used as the immunogen for this anti-ACTH antibody.

Storage

Store the anti-ACTH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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