ALOX12 Antibody / 12S-lipoxygenase (RQ8685)

ALOX12 Antibody Human, Rat and Mouse WB. Western blot testing of 1) human A549, 2) human MCF7, 3) human Jurkat, 4) rat RH35, and 5) mouse RAW264.7 cell lysates with ALOX12 antibody. A distinct immunoreactive band is detected at approximately 76 kDa in all samples, consistent with the predicted molecular weight of 12S-Lipoxygenase (ALOX12). The results demonstrate cross-species detection of this arachidonic acid-metabolizing enzyme, which generates bioactive lipid mediators involved in platelet activation, inflammatory signaling, oxidative stress, and regulation of cellular responses.

ALOX12 Antibody Human, Rat and Mouse WB. Western blot testing of 1) human HeLa, 2) human A431, 3) human K562, 4) rat spleen, 5) rat RH35, 6) mouse spleen, and 7) mouse RAW264.7 cell lysates with ALOX12 antibody at 0.5 ug/ml. A prominent immunoreactive band is detected at approximately 76 kDa, consistent with the predicted molecular weight of 12S-Lipoxygenase (ALOX12). The results demonstrate cross-species detection of this lipid-metabolizing enzyme, which catalyzes the oxygenation of arachidonic acid to generate bioactive lipid mediators involved in platelet function, inflammation, oxidative stress, and cellular signaling.

ALOX12 Antibody A549 Cell IF. Immunofluorescent staining of A549 cells with ALOX12 antibody (green). Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Nuclei are counterstained with DAPI (blue). ALOX12 immunoreactivity is observed predominantly within the cytoplasm, consistent with the expected localization of 12S-Lipoxygenase (ALOX12), an arachidonic acid-metabolizing enzyme that catalyzes the formation of bioactive lipid mediators involved in platelet activation, inflammatory signaling, oxidative stress, and regulation of cellular responses.