AKR1B1 Antibody / Aldose reductase [clone CPTC-AKR1B1-2] (V3987)

IHC testing of FFPE human prostate with AKR1B1 antibody (clone CPTC-AKR1B1-2). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AKR1B1 antibody (clone CPTC-AKR1B1-2). These results demonstrate the foremost specificity of the CPTC-AKR1B1-2 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free AKR1B1 antibody (clone CPTC-AKR1B1-2) as confirmation of integrity and purity.

Western blot testing of human HeLa cell lysate with AKR1B1 antibody (clone CPTC-AKR1B1-2). Predicted molecular weight ~36 kDa.

Immunofluorescent staining of human A549 cells with AKR1B1 antibody (clone CPTC-AKR1B1-2, green) and DAPI nuclear stain (blue).