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Home >> Antibodies >> AFP Antibody / Alpha Fetoprotein

AFP Antibody / Alpha Fetoprotein [clone C3] (V2002)

  Catalog No Formulation Size Price (USD)  
V2002-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2002-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2002SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2002IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human fetal liver stained with AFP antibody (C3).
SDS-PAGE Analysis of Purified, BSA-Free AFP Antibody (clone C3). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human, Monkey, Dog, and Pig. It does not react with Cow, Cat, Mouse, and Rat.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name C3
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 174
Localization Cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Alpha Fetoprotein / AFP antibody is available for research use only.

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Description

This antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.

Storage

Store the AFP antibody at 2-8°C.

Alternate Names

Alpha fetoglobulin; FETA; HPAFP, Alpha fetoprotein, AFP antibody

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