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Home >> Antibodies >> Actin Antibody

Actin Antibody (R30386)

  Catalog No Formulation Size Price (USD)  
R30386 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 285
Western blot with Actin antibody at 0.5ug/ml and 40ug of lysate; Lane 1: rat kidney; 2: A549; 3: MCF-7; 4: HEPG2
IHC-P: Actin antibody testing of human breast cancer tissue
IHC-P: Actin antibody testing of human intestinal cancer tissue
IHC-P: Actin antibody testing of mouse intestine
IHC-P: Actin antibody testing of mouse spleen
IHC-P: Actin antibody testing of rat intestine
IHC-P testing of rat spleen
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P63261
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml (1)
Limitations This product is available for research use only.

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Description

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. There are three isoforms: alpha, beta, and gamma. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. HIER: steamed with pH6 citrate buffer is required for FFPE testing.

Immunogen

Amino acids 39-51 (RHQGVMVGMGQKD) was used as the immunogen for this Actin antibody (100% homologous human, mouse and rat).

Storage

After reconstitution, the Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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