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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Actin Antibody

Actin Antibody (R30385)

  Catalog No Formulation Size Price (USD)  
R30385 0.2mg/ml in PBS with 50% glycerol and 0.025% sodium azide 100 ug 285
Western blot testing of Actin antibody and Lane 1: rat cardiac muscle; 2: rat brain; 3: rat testis; 4: rat skeletal muscle; 5: MM231 lysate; 6: HeLa lysate; 7: SMMC-7721 lysate; 8: HT1080 lysate; 9: SW620 lysate
IHC-P: Actin antibody testing of rat heart tissue. HIER: steamed with pH6 citrate buffer.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
UniProt P63261
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Actin antibody is available for research use only.

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Description

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of the human gamma isoform (SGPSIVHRKCF) was used as the immunogen for this Actin antibody (100% homologous human, mouse and rat).

Storage

Store the Actin antibody at -20oC.

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