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Home >> Antibodies >> p504S Antibody (Recombinant)

p504S Antibody (Recombinant) [clone 13H4] (V2505S)

  Catalog No Formulation Size Price (USD)  
V2505S-0.5ML Bioreactor concentrate with 0.05% sodium azide 0.5 ml 379

V2505S-0.1ML Bioreactor concentrate with 0.05% sodium azide 0.1 ml 199

V2505IHC-7ML Prediluted in 1X PBS, 0.05% sodium azide; *For IHC use only* 7 ml 329
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p504S antibody (13H4)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p504S antibody (13H4)
Availability 1-3 business days
Species Reactivity Human. Other species not known.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 13H4
Purity Unpurified high titer supernatant
UniProt Q9UHK6
Localization Cytoplasmic
Applications Western blot : published application
Immunohistochemistry (FFPE) : 1:50-1:100 for 30-60 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This p504S antibody is available for research use only.

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Description

This antibody recognizes a protein of 44kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Application Notes

Optimal dilution of the p504S antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length human recombinant protein was used as the immunogen for the p504S antibody.

Storage

Store the p504S antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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