CD45RO Antibody UCHL-1 | V2237 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2237-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2237-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2237SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2237IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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CD45RO antibody UCHL-1 immunohistochemistry tonsil

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IHC testing of human tonsil stained with CD45RO antibody (UCHL-1).

FACS staining of human lymphocytes using CD45RO antibody (UCHL-1, red) and isotype control (green).

SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone UCHL-1). Confirmation of Integrity and Purity of the Antibody.

Immunofluorescent staining of FFPE human tonsil tissue with CD45RO antibody (clone UCHL-1).

Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody; Red=isotype control, Blue= CD45RO antibody.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2a, kappa
Clone Name	UCHL-1
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	5788
Localization	Cell surface
Applications	Flow cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations	This CD45RO antibody is available for research use only.
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Description

This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.