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Home >> Antibodies >> Podocalyxin Antibody

Podocalyxin Antibody [clone 3D3] (V2227)

  Catalog No Formulation Size Price (USD)  
V2227-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2227-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2227SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2227IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of angiosarcoma stained with Podocalyxin antibody.
Western blot testing of human samples
FACS surface staining of NCCIT cells with Podocalyxin antibody
IHC testing of FFPE human placenta with Podocalyxin antibody
Species Reactivity Human, Rabbit, Rat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 3D3
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 5420
Localization Cell surface and cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Podocalyxin antibody is available for research use only.

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Description

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.

Storage

Store the Podocalyxin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx

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