Insulin Antibody [clone 2D11-H5 or INS05] (V2156)

Description

This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. antibody to insulin is an important beta-cell and insulinoma marker.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.

Storage

Store the Insulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

IDDM2, ILPR, IRDN, MODY10, Proinsulin, Insulin antibody