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Home >> Antibodies >> MART-1 Antibody

MART-1 Antibody [clone M2-7C10] (V2116)

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  Catalog No Formulation Size Price (USD)  
Image V2116-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2116-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2116SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2116IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human skin stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free MART-1 Antibody (clone M2-7C10). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, lambda
Clone Name M2-7C10
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2315
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This MART-1 antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human MART-1 protein was used as the immunogen for this antibody.

Storage

Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, MART-1 antibody, Melan-A antibody

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