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Home >> Antibodies >> CD34 Antibody

CD34 Antibody [clone QBEnd/10] (V2067)

  Catalog No Formulation Size Price (USD)  
V2067-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2067-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2067SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2067IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human tonsil (10X) stained with CD34 antibody (QBEnd/10).
IHC testing of human tonsil (20X) stained with CD34 antibody (QBEnd/10).
Species Reactivity Human, Cynomolgus Monkey, Rhesus Monkey. Does not react with Rat, Sheep, Cow and Dog. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name QBEnd/10
Purity Protein G purified antibody
Buffer 1X PBS, pH 7.4
Gene ID 947
Localization Cell surface
Applications Flow Cytometry : use antibody at 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This CD34 antibody is available for research use only.

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Description

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85% of angiosarcoma and Kaposi’s sarcoma, but with a lower specificity.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD34 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Storage

Store the CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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