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  • Email: info@nsjbio.com
Home >> Antibodies >> CD6 Antibody

CD6 Antibody [clone 3F7B5] (V2039)

  Catalog No Formulation Size Price (USD)  
V2039-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2039-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2039SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2039IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human tonsil stained with CD6 antibody (3F7B5).
Formalin/paraffin human tonsil stained with CD6 antibody (3F7B5).
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 3F7B5
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 923
Localization Cell surface and cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This CD6 antibody is available for research use only.

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Description

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. It shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. CD6 antibody can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The human rheumatoid synovial T cell line ST-1 was used as the immunogen for this CD6 antibody.

Storage

Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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