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Home >> Antibodies >> CD147 Antibody

CD147 Antibody [clone 8D6] (V2015)

  Catalog No Formulation Size Price (USD)  
V2015-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2015-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2015SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2015IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of FFPE human renal cell carcinoma with CD147 antibody (clone 8D6).
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 8D6
Purity Protein G purified CD147 antibody
Buffer 1X PBS, pH 7.4
Gene ID 682
Localization Cell surface, cytoplasmic
Applications Functional Activity : Inhibits homotypic aggregation and adhesion to matrix proteins
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1:100-1:200
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This CD147 antibody is available for research use only.

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Description

This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.

Storage

Store the CD147 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor, CD147 antibody

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