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Home >> Antibodies >> Bcl2 Antibody Cocktail

Bcl2 Antibody Cocktail [clone 100/D5 + 124] (V2010)

  Catalog No Formulation Size Price (USD)  
V2010-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2010-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2010SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2010IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
FFPE non-Hodgkin's lymphoma stained with Bcl2 antibody cocktail.
Western blot testing of human samples using Bcl2 antibody cocktail.
FACS staining (intracellular) of Jurkat cells using Bcl2 antibody cocktail (red) and isotype control (green).
Species Reactivity Human. Does not react with Mouse and Rat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa (100/D5 + 124)
Clone Name 100/D5 + 124
Purity Protein G purification
Buffer 1X PBS, pH 7.4
Gene ID 596
Localization Cytoplasmic, membrane
Applications Flow Cytometry : 0.5-1ug/million cells in 0.1ml
Immunofluorescence : 0.5-1ug/ml
Western blot : 0.5-1ug/ml
Immunoprecipitation : 0.5-1µg/500ug protein lysate
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Bcl2 antibody cocktail is available for research use only.

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Description

B-cell lymphoma 2 is an oncogene that regulates apoptosis, having both inducing and inhibiting functions. In cancer cells, Bcl2 binds pro-apoptotic proteins, preventing programmed cell death from occurring. Overexpression is seen in many cancers including lung, breast, prostate and lymphomas. In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Application Notes

Variations in protocols, secondaries and substrates may require the antibody cocktail to be titered for optimal performance.

1. FFPE staining is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.

Storage

Store the Bcl2 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

B-cell lymphoma 2, Bcl2 antibody cocktail

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