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Home >> Antibodies >> Bax Antibody

Bax Antibody [clone 2D2] (V2005)

  Catalog No Formulation Size Price (USD)  
V2005-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2005-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2005SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2005IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of Hodgkin's lymphoma stained with Bax antibody (Clone 2D2).
Species Reactivity Human and Monkey. Does not react with Mouse and Rat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 2D2
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 581
Localization Cytoplasmic
Applications ELISA : order BSA-free format for coating
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Bax antibody is available for research use only.

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Description

This antibody recognizes a protein of 21kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Storage

Store the Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

BCL2 associated X

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